Figure 4

Whole-cell recordings of dentate granule cells show that PAR1 activation causes depolarization in an individual neuron. A) Image of a visually-identified dentate granule cell, patched under whole-cell configuration. Scale bar is 10 μm. B) The cell was injected with current as indicated to induce action potentials. Current was injected from -60 pA to 60 pA. C) The cell was subsequently challenged with 30 μM TFLLR, which induced a slow depolarization that drove the cell to fire action potentials repetitively. D) Out of 14 cells, the 7 neurons that showed spike firing activity had an average depolarization of 11.5 ± 3.5 mV (mean ± SEM), induced by TFLLR. The internal solution contained K-gluconate (see Methods).