Figure 4

The number of proliferating type 1 and 2a cells was greater in the galectin-1 -null mutant DG than the control DG. (A-A'''') High-magnification images of Ki67 (blue), FABP7 (green), and DCX (red) triple staining with nuclear Hoechst (white) in the DG. Arrow indicates a Ki67+/FABP7+/DCX- cell. (B) The total number of Ki67+ cells was not different between the galectin-1 heterozygous and galectin-1-null mice. P = 0.25; n > 5 mice each. (C) The number of Ki67+/FABP7+/DCX- cells was higher in the galectin-1 null mutants, suggesting that Galectin-1 negatively regulates the proliferation of type 1 and 2a cells in the adult DG. P < 0.05; n > 5 mice each. P < 0.01; n > 5 mice each. (D-D'''') High-magnification images of cv-Casp3 (blue), FABP7 (green), and DCX (red) triple staining, with nuclear Hoechst (white) in the DG. Arrow indicates a cv-Casp3+/FABP7+/DCX- cell. (E) The number of cv-Casp3+ cells was comparable between the galectin-1 heterozygous and KO mice. P = 0.12; n > 11 mice each. (G) No significant difference between the number of cv-Casp3+/FABP7+/DCX- cells in the SGZ of galectin-1 KO and control mice. n > 22 mice each. (Scale bars: A-A'''', D-F'''', 5 μm)