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Figure 4 | Molecular Brain

Figure 4

From: Direct interaction between GluR2 and GAPDH regulates AMPAR-mediated excitotoxicity

Figure 4

Regulation of the AMPAR-mediated cell death in cultured neurons and OGD model. A, Bar graph summarizing the quantitative measurements of PI fluorescence from rat hippocampal primary culture with KA treatment (100 μM, 1 hr) in the presence/absence of the GluR2NT1-3–2 peptide. ***Significantly different from KA group (P < 0.001, n = 9 per group), t-test. B, Bar graph summarizing the quantitative measurements of PI fluorescence from rat hippocampal primary culture with OGD protocol in the presence/absence of the GluR2NT1-3–2 peptide. *Significantly different from OGD group (P < 0.05, n = 9 per group), t-test. C, Western blot analysis of GAPDH (upper panel) and GluR2 (lower panel) expression in HEK 293 T cells expressing GluR1/2 subunits in the presence/absence of the GAPDH siRNA. D, Bar graph summarizing the quantitative measurements of PI fluorescence from HEK-293 T cells expressing GluR1/2 subunits with glutamate treatment in the presence/absence of the GAPDH siRNA (P < 0.05, n = 9 per group), t-test. E, Propidim iodide positive cells (red) from HEK-293 T expressing GluR1/2 subunits with glutamate treatment in the presence/absence of the GAPDH siRNA, scale bar 50 μm. All PI fluorescence measurement assays were performed 3 times independently.

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