Gliosis and lipofuscin accumulation in Hdh140Q/3xFlag20Qand Hdh140Q/3xFlag7Qmice. (A) Images of the striatum of 2 year-old wild type (+/+), Hdh 140Q/3xFlag7Q, Hdh140Q/3xFlag20Q, and Hdh 140Q/3xFlag140Q mice probed with antibodies against GFAP (green) and htt inclusions (MW8, red). Nuclei were visualized with To-Pro-3 (blue). (B) The mean total pixel area of GFAP staining per microscopic field of the ventral striatum was plotted for each genotype (n=3 mice for each genotype). (C) Images of lipofuscin autofluorescence (yellow) in the striatum of 2 year-old wild type (+/+), Hdh 140Q/3xFlag7Q, Hdh 140Q/3xFlag20Q, and Hdh 140Q/3xFlag140Q mice. Nuclei were stained with To-Pro-3. (D) The mean total pixel area of lipofuscin autofluorescence was plotted for each genotype (n=3 mice for each genotype, mean ± SEM plotted). *P<0.05, **P<0.01 in B,D. Scale bar = 25 μm in A,C.