Wnt proteins induce AChR clusters in muscle cells. A, C2 myotubes were stimulated with conditioned media containing Wnt proteins or control medium for 16 h. AChR clusters were visualized by R-BTX staining and indicated by arrows. B. Quantification data of A. AChR clusters greater than 4 μm in length were quantified. *, p < 0.01, Student's t test. C, Wnt mRNAs are expressed in muscle. Total RNAs were extracted from skeletal muscles of P0, P15, P30 and adult mice and used for qRT-PCR. Relative expression was shown in histograms.