Figure 1

Surface trafficking and total expression are the same for A39V-Cav1.2-HA and WT-Cav1.2-HA in the presence of β2b. (A). β2b significantly increases the surface trafficking of A39V-Cav1.2-HA and WT-Cav1.2-HA in nonpermeablized tsA-201 cells. (B). Quantification of HA surface pool displayed as fluorescence per cell (arbitrary light units). β2b and β1b (Additional file 2: Figure S2A) significantly increase the fluorescence per cell of WT-Cav1.2-HA, while only β2b significantly increased the surface fluorescence of A39V-Cav1.2-HA (*p = < 0.05 and #p = < 0.05 by one-way ANOVA). Cavβ1b does not significantly increase the fluorescence per cell of A39V-Cav1.2 (p = > 0.05 by one-way ANOVA). (C). Total protein expression of A39V-Cav1.2-HA and WT-Cav1.2-HA from tsA-201 cell lysates expressed with and without β2b. (D). Quantification of A39V-Cav1.2-HA and WT-Cav1.2-HA total expression (integrated density units) with and without β2b/β1b (Additional file 2: Figure S2B). The data is expressed as a ratio of HA/α-actin. Both β2b and β1b significantly increase the expression of A39V-Cav1.2 (*p = < 0.05 by one-way ANOVA). A39V-Cav1.2 shows less expression than WT-Cav1.2 in the absence of Cavβ (#p = 0.04 student's t-test).