| |
Current Density (pA/pF)
|
V1/2 Activation (mV)
|
Slope of Activation (mV)
|
V1/2 Steady State Inactivation (mV)
|
Slope of Steady State Inactivation (mV)
|
|---|
|
Cav1.2 + Cavα2δ
|
-4.4 +/- 0.5
|
-9.1 +/- 1.3
|
4.7 +/- 0.7
|
-5.4 +/-1.8
|
6.9 +/- 0.6#
|
|
A39V-Cav.2 + Cavα2δ
|
-3.3 +/- 0.5
|
-11.7 +/- 1.5
|
4.6 +/- 0.9
|
-7.3 +/- 1.9
|
11.8 +/- 1.4#
|
|
Cav1.2 + α2δ + Cavβ2b
|
-9.1 +/- 1.4 *
|
-3.8 +/- 0.8
|
10.2 +/- 1.4
|
-7.1 +/- 1.7
|
11.1 +/- 1.8
|
|
A39V-Cav1.2 + Cavα2δ + Cavβ2b
|
-9.5 +/- 1.3 **
|
-3.0 +/- 1.4
|
13.0 +/- 2.0
|
-5.2 +/- 2.8
|
11.5 +/- 1.2
|
|
Cav1.2 + Cavα2δ + Cavβ1b
|
-10.6 +/- 1.1 *
|
-9.0 +/- 0.8
|
8.8 +/- 1.2
|
-6.3 +/- 1.1
|
8.4 +/- 1.2
|
|
A39V-Cav1.2 + Cavα2δ + Cavβ1b
|
-8.3 +/- 1.0 **
|
-6.4 +/- 1.1
|
8.1 +/- 0.9
|
-6.8 +/- 1.6
|
9.3 +/- 0.8
|
- - * indicates significant increase in current density when compared to Cav1.2 without a Cavβ subunit; p = < 0.05, ANOVA
- - ** indicates significant increase in current density when compared to A39V-Cav1.2 without a Cavβ subunit; p = < 0.05, ANOVA
- - Data expressed as mean +/- SEM. Current density was determined from a minimum of 24 cells per condition, from three separate transfections. All other values determined from aforementioned cells which fit the modified Boltzmann, or Boltzmann equations (> 11 cells/per condition)
- - # indicates significant difference in slope of steady state inactivation; p = > 0.01, Students T-test
