The Brugada syndrome mutation A39V does not affect surface expression of neuronal rat Cav1.2 channels

Table 1 Current densities and voltage-dependent properties of A39V-Cav1.2 without Cavβ, with Cavβ2b, or with Cavβ1b

	Current Density (pA/pF)	V_1/2 Activation (mV)	Slope of Activation (mV)	V_1/2 Steady State Inactivation (mV)	Slope of Steady State Inactivation (mV)
Cav1.2 + Cavα2δ	-4.4 +/- 0.5	-9.1 +/- 1.3	4.7 +/- 0.7	-5.4 +/-1.8	6.9 +/- 0.6#
A39V-Cav.2 + Cavα2δ	-3.3 +/- 0.5	-11.7 +/- 1.5	4.6 +/- 0.9	-7.3 +/- 1.9	11.8 +/- 1.4#
Cav1.2 + α2δ + Cavβ2b	-9.1 +/- 1.4 *	-3.8 +/- 0.8	10.2 +/- 1.4	-7.1 +/- 1.7	11.1 +/- 1.8
A39V-Cav1.2 + Cavα2δ + Cavβ2b	-9.5 +/- 1.3 **	-3.0 +/- 1.4	13.0 +/- 2.0	-5.2 +/- 2.8	11.5 +/- 1.2
Cav1.2 + Cavα2δ + Cavβ1b	-10.6 +/- 1.1 *	-9.0 +/- 0.8	8.8 +/- 1.2	-6.3 +/- 1.1	8.4 +/- 1.2
A39V-Cav1.2 + Cavα2δ + Cavβ1b	-8.3 +/- 1.0 **	-6.4 +/- 1.1	8.1 +/- 0.9	-6.8 +/- 1.6	9.3 +/- 0.8

- * indicates significant increase in current density when compared to Cav1.2 without a Cavβ subunit; p = < 0.05, ANOVA
- ** indicates significant increase in current density when compared to A39V-Cav1.2 without a Cavβ subunit; p = < 0.05, ANOVA
- Data expressed as mean +/- SEM. Current density was determined from a minimum of 24 cells per condition, from three separate transfections. All other values determined from aforementioned cells which fit the modified Boltzmann, or Boltzmann equations (> 11 cells/per condition)
- # indicates significant difference in slope of steady state inactivation; p = > 0.01, Students T-test

ISSN: 1756-6606