Decrease in adult hippocampal neurogenesis in mutant mice. (A, B) Cell proliferation was analyzed using BrdU staining (n = 6 mice each). Arrowheads indicate BrdU-positive cells. (C, D) Images of doublecortin (DCX)-positive cells (arrowheads) in the GCL are shown. ML, molecular layer. SGZ, subgranular zone. HL, hilus. (E) Quantification of DCX-positive cell numbers in the GCL (n = 4 mice each). (F) The positions of DCX-positive cells are shown as relative values between the subgranular layer (SGL; 0) and the border with the molecular layer (ML; 1) (n = 4 mice each).