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Figure 4 | Molecular Brain

Figure 4

From: Fluorometric immunocapture assay for the specific measurement of matrix metalloproteinase-9 activity in biological samples: application to brain and plasma from rats with ischemic stroke

Figure 4

MMP-9 activity correlates in gelatin-substrate zymography and FRET peptide-based immunocapture assay. Results of MMP-9 activity from rat MCAO brain tissue homogenate samples were compared between zymography and the FRET immunocapture assay. (A) Representative zymogram of 50 μg total protein from rat cerebral cortex homogenates, ipsilateral (ipsi) and contralateral (contra) hemispheres, and HT-1080 conditioned media. MMP-9 (94 and 88 kDa) and MMP-2 (72 kDa) are shown. Quantification of MMP-9 included the 94 and 88 kDa bands. (B) Correlation of relative lysis units (RLUs) quantified from zymography and relative fluorescent units (RFUs) recorded from the FRET peptide-based assay from 16 rat ipsilateral and 16 rat contralateral cerebral cortex samples. Compared with linear regression analysis, R2 = 0.8024, p < 0.0001, n = 32.

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Molecular Brain

ISSN: 1756-6606