Figure 5
From: Delta-like 1 regulates Bergmann glial monolayer formation during cerebellar development
Ectopic BG are eliminated by apoptosis in Dll1(loxP/loxP)/hGFAP-Cre mice. (A-D) TUNEL staining of Dll1(loxP/+)/hGFAP-Cre (A, C) and Dll1(loxP/loxP)/hGFAP-Cre (B, D) mice at P5 (A, B) and P7 (C, D). The black arrowheads in (A-D) show TUNEL-positive cells. (E, F) Immunostaining for GLAST (green) and TUNEL (red) in Dll1(loxP/+)/hGFAP-Cre (E) and Dll1(loxP/loxP)/hGFAP-Cre (F) mice at P5. The white arrows indicate TUNEL-positive and GLAST-negative cells. The white arrowheads indicate TUNEL-positive and GLAST-positive cells in the EGL. (G-J) Quantification of the number of TUNEL-positive and GLAST-negative cells (G, I) and TUNEL-positive and GLAST-positive cells in the EGL (H, J) at P5 (G, H) and P7 (I, J). Scale bars: 50 μm (A-D) 20 μm (E-F). EGL, external granular layer; PCL, Purkinje cell layer.