Table 1 Summary of systematic behavioral tests for Sept4 Tg/+ mice in comparison with Sept4 −/− mice
Tests | Mental/physical activities | Indices measured | Alteration from wildtype | |
|---|---|---|---|---|
(inexhaustive) | Sept4tg/+(tg) | Sept4−/−(ko) | ||
Table 1. Summary of systematic behavioral tests for Sept4Tg/+ (tg) mice in comparison with Sept4−/− (ko) mice (Additional Figure A1) | General health | Body weight | → | → |
Rectal temperature | → | → | ||
Grip strength | → | → | ||
Hanging persistence | → | → | ||
Light/dark transition test | Exploratory activity | Distance traveled in the light chamber | → | → |
(Additional Figure A2) | Light avoidance | Distance traveled in the dark chamber | → | → |
Latency to the first entry to the light chamber | → | → | ||
Time stayed in the light chamber | → | → | ||
Number of transitions between chambers | → | → | ||
Open field test | Exploratory activity | Distance traveled | →* | → |
(Figure 3) | Avoidance from open space | Center time | → | → |
Open field test + metamphetamine | Induced surge of exploratory activity | Distance traveled | →* | ↓ |
(Figure 4) | Center time | → | N.D. | |
Elevated plus maze test | Exploratory activity | Distance traveled | → | → |
(Additional Figure A3) | Height avoidance | Entries into open arms | ↑ | → |
Number of entries | → | → | ||
Time stayed on open arms | → | → | ||
Acoustic startle response | Startle reflex to loudness | Ampliture of body motion | → | → |
(Additional Figure A4A) | ||||
Prepulse inhibition (PPI) test | Sensorimotor gating | Decrement of startle amplitude | → | ↑ |
(Additional Figure A4B) | ||||
Porsolt forced swim test | Despair-like behavior | Latency to immobility | → | → |
(Additional Figure A5) | ||||
Home cage monitoring | Diurnal cycle of locomotor activity | Activity level (distance traveled) | → (basal) | → |
(Figure 5) | ↓(at surges) | → | ||
Social interaction test | Social behavior, anxiety-like behavior | Distance traveled | → | → |
(1 chamber, stranger pair) | Number of contacts | → | → | |
(Additional Figure A7) | Total duration of active contacts | → | → | |
Mean contact duration | → | → | ||
Total duration of contacts | → | → | ||
Social interaction test | Social behavior, anxiety-like behavior | Time spent with novel stranger | ↓(Step 2) | N.D. |
(3 chamber, 1–2 caged strangers) | ||||
(Figure 6) | Distance traveled | ↓(Step 2) | N.D. | |
Rota rod test | Motor coordination/learning | Latency to fall | → | → |
(Additional Figure A6) | ||||
Beam test | Motor coordination/learning | Moving speed | N.D. | → |
Gait analysis | Mechanics of limb movement | Limb positions and timings | → (front paw angle) | N.D. |
(Additional Figure A8) | ↓ (hind paw angle) | N.D. | ||
Hot plate test | Aversive response to noxious stimulus | Latency to limb withdrawal | → | → |
(Additional 1 Figure A9) | ||||
Tail suspension test | Behavioral despair | Latency to immobility | → | → |
(Additional 1 Figure A10) | ||||
Fear conditioning test | Fear memory | Conditioning | → | → |
(Additional 1 Figure A11) | Contextual testing | → | → | |
Cued test with altered context | → | → | ||