| | |
(inexhaustive)
|
Sept4tg/+(tg)
|
Sept4−/−(ko)
|
|
Table 1. Summary of systematic behavioral tests for Sept4Tg/+ (tg) mice in comparison with Sept4−/− (ko) mice (Additional Figure A1)
|
General health
|
Body weight
|
→
|
→
|
|
Rectal temperature
|
→
|
→
|
|
Grip strength
|
→
|
→
|
|
Hanging persistence
|
→
|
→
|
|
Light/dark transition test
|
Exploratory activity
|
Distance traveled in the light chamber
|
→
|
→
|
|
(Additional Figure A2)
|
Light avoidance
|
Distance traveled in the dark chamber
|
→
|
→
|
|
Latency to the first entry to the light chamber
|
→
|
→
|
|
Time stayed in the light chamber
|
→
|
→
|
|
Number of transitions between chambers
|
→
|
→
|
|
Open field test
|
Exploratory activity
|
Distance traveled
|
→*
|
→
|
|
(Figure 3)
|
Avoidance from open space
|
Center time
|
→
|
→
|
|
Open field test + metamphetamine
|
Induced surge of exploratory activity
|
Distance traveled
|
→*
|
↓
|
|
(Figure 4)
| |
Center time
|
→
|
N.D.
|
|
Elevated plus maze test
|
Exploratory activity
|
Distance traveled
|
→
|
→
|
|
(Additional Figure A3)
|
Height avoidance
|
Entries into open arms
|
↑
|
→
|
|
Number of entries
|
→
|
→
|
|
Time stayed on open arms
|
→
|
→
|
|
Acoustic startle response
|
Startle reflex to loudness
|
Ampliture of body motion
|
→
|
→
|
|
(Additional Figure A4A)
| | | | |
|
Prepulse inhibition (PPI) test
|
Sensorimotor gating
|
Decrement of startle amplitude
|
→
|
↑
|
|
(Additional Figure A4B)
| | | | |
|
Porsolt forced swim test
|
Despair-like behavior
|
Latency to immobility
|
→
|
→
|
|
(Additional Figure A5)
| | | | |
|
Home cage monitoring
|
Diurnal cycle of locomotor activity
|
Activity level (distance traveled)
|
→ (basal)
|
→
|
|
(Figure 5)
| | |
↓(at surges)
|
→
|
|
Social interaction test
|
Social behavior, anxiety-like behavior
|
Distance traveled
|
→
|
→
|
|
(1 chamber, stranger pair)
|
Number of contacts
|
→
|
→
|
|
(Additional Figure A7)
|
Total duration of active contacts
|
→
|
→
|
|
Mean contact duration
|
→
|
→
|
|
Total duration of contacts
|
→
|
→
|
|
Social interaction test
|
Social behavior, anxiety-like behavior
|
Time spent with novel stranger
|
↓(Step 2)
|
N.D.
|
|
(3 chamber, 1–2 caged strangers)
| | | | |
|
(Figure 6)
| |
Distance traveled
|
↓(Step 2)
|
N.D.
|
|
Rota rod test
|
Motor coordination/learning
|
Latency to fall
|
→
|
→
|
|
(Additional Figure A6)
| | | | |
|
Beam test
|
Motor coordination/learning
|
Moving speed
|
N.D.
|
→
|
|
Gait analysis
|
Mechanics of limb movement
|
Limb positions and timings
|
→ (front paw angle)
|
N.D.
|
|
(Additional Figure A8)
| | |
↓ (hind paw angle)
|
N.D.
|
|
Hot plate test
|
Aversive response to noxious stimulus
|
Latency to limb withdrawal
|
→
|
→
|
|
(Additional 1 Figure A9)
| | | | |
|
Tail suspension test
|
Behavioral despair
|
Latency to immobility
|
→
|
→
|
|
(Additional 1 Figure A10)
| | | | |
|
Fear conditioning test
|
Fear memory
|
Conditioning
|
→
|
→
|
|
(Additional 1 Figure A11)
|
Contextual testing
|
→
|
→
|
| | |
Cued test with altered context
|
→
|
→
|
