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Table 1 Summary of systematic behavioral tests for Sept4 Tg/+ mice in comparison with Sept4 −/− mice

From: Chronic overload of SEPT4, a parkin substrate that aggregates in Parkinson’s disease, causes behavioral alterations but not neurodegeneration in mice

Tests Mental/physical activities Indices measured Alteration from wildtype
   (inexhaustive) Sept4tg/+(tg) Sept4−/−(ko)
Table 1. Summary of systematic behavioral tests for Sept4Tg/+ (tg) mice in comparison with Sept4−/− (ko) mice (Additional Figure A1) General health Body weight
Rectal temperature
Grip strength
Hanging persistence
Light/dark transition test Exploratory activity Distance traveled in the light chamber
(Additional Figure A2) Light avoidance Distance traveled in the dark chamber
Latency to the first entry to the light chamber
Time stayed in the light chamber
Number of transitions between chambers
Open field test Exploratory activity Distance traveled →*
(Figure 3) Avoidance from open space Center time
Open field test + metamphetamine Induced surge of exploratory activity Distance traveled →*
(Figure 4)   Center time N.D.
Elevated plus maze test Exploratory activity Distance traveled
(Additional Figure A3) Height avoidance Entries into open arms
Number of entries
Time stayed on open arms
Acoustic startle response Startle reflex to loudness Ampliture of body motion
(Additional Figure A4A)     
Prepulse inhibition (PPI) test Sensorimotor gating Decrement of startle amplitude
(Additional Figure A4B)     
Porsolt forced swim test Despair-like behavior Latency to immobility
(Additional Figure A5)     
Home cage monitoring Diurnal cycle of locomotor activity Activity level (distance traveled) → (basal)
(Figure 5)    ↓(at surges)
Social interaction test Social behavior, anxiety-like behavior Distance traveled
(1 chamber, stranger pair) Number of contacts
(Additional Figure A7) Total duration of active contacts
Mean contact duration
Total duration of contacts
Social interaction test Social behavior, anxiety-like behavior Time spent with novel stranger ↓(Step 2) N.D.
(3 chamber, 1–2 caged strangers)     
(Figure 6)   Distance traveled ↓(Step 2) N.D.
Rota rod test Motor coordination/learning Latency to fall
(Additional Figure A6)     
Beam test Motor coordination/learning Moving speed N.D.
Gait analysis Mechanics of limb movement Limb positions and timings → (front paw angle) N.D.
(Additional Figure A8)    ↓ (hind paw angle) N.D.
Hot plate test Aversive response to noxious stimulus Latency to limb withdrawal
(Additional 1 Figure A9)     
Tail suspension test Behavioral despair Latency to immobility
(Additional 1 Figure A10)     
Fear conditioning test Fear memory Conditioning
(Additional 1 Figure A11) Contextual testing
   Cued test with altered context
  1. Comparative table of systematic behavioral test results with Sept4Tg/+ (tg) and wild-type littermate male mice (C57BL/6, this study) and with Sept4−/− (ko) and wild-type littermate male mice (C57BL/6J, [12]). Symbols: →, no significant difference (p > 0.05); ↑, statistically significant increase; ↓, statistically significant decrease; in comparison with wildtype littermates. N.D., not determined.