Decay kinetics of fast rising mEPSCs was not changed by PAR1 activation. A. Representative superimposed, normalized, averaged mEPSC traces (gray) from fast rising mEPSCs in scrambled shRNA or Best1 shRNA infected slices. The average traces from basal condition (Before; green), TFLLR treated group (TFLLR; red), and TFLLR + APV-treated group (APV; blue) were best fitted with a single component exponential function. B. Example of decay fitting by a single exponential function. Fast rising mEPSCs from TFLLR treated hippocampal slices was fitted well by a single component exponential function. Red line indicates decay fitting by a single exponential decay function. NMDAR component is not detected in fast rising mEPSCs. C. Bar graphs represent single component of decay tau of fast rising mEPSCs before TFLLR (green), after TFLLR (red), and after TFLLR + APV (blue) treatment in naïve, scrambled shRNA injected naïve, Best1 shRNA injected hGFAP-CreERT2, or Best1 shRNA injected CaMKIIα-Cre mice, respectively. Sc, scrambled shRNA. B1, Best1 shRNA. Tam, tamoxifen. Numbers of tested slices from at least three independent mice are indicated.