An appearance of NMDAR component in slowly rising mEPSCs by PAR1 activation. A. Representative superimposed, normalized, averaged mEPSC traces (gray) from slowly rising mEPSCs in scrambled shRNA or Best1 shRNA infected slices. The average traces from basal condition (left, Before; green) and TFLLR + APV treated group (APV; blue) were best fitted with a single component exponential function. But mEPSC traces from TFLLR-treated group (TFLLR; red) were best fitted with a two component exponential function, which is impaired by Best1 shRNA expression (right). B. Example of decay fitting by two component exponential functions in TFLLR treated slices. Red line is decay fitting by single exponential function, and blue line is decay fitting by second exponential decay function. NMDAR component is detected in slowly rising mEPSCs generated in distal dendrite. C. Bar graphs represent fast component of decay tau of slowly rising mEPSCs. Tau 1 was not significantly changed by Best1-mediated astrocytic glutamate. D. Summary of slow component of decay tau of slowly rising mEPSCs. N.D. indicates not detected by a two exponential function fitting. E. Schematic diagram of fast rising and slowly rising mEPSCs in the presence of TFLLR. NMDAR component is not detected in fast rising mEPSCs generating at the proximal dendrites clamped well, whereas activation of NMDAR by TFLLR can be detected at the distal dendrites because of poor voltage clamp.