Figure 2

Immunocytochemical staining for the IL-1 type 1 receptor and P-STAT3-Tyr705 in control neurons. DRG sensory neurons from normal rats were cultured for 1 day in the presence of low dose of neurotrophic growth factors and were fixed and immunostained with (a, g) no primary antibody, or stained for (b) IL-1 type 1 receptor antibody or (c) β-tubulin III or (h) P-STAT3-Tyr705 or (i) β-tubulin III. Cultures were also treated for 24 h with IL-1β (10 ng/ml) and stained for (d) IL-1 type 1 receptor or (e) β-tubulin III or (j) P-STAT3-Tyr705 or (k) β-tubulin III. (f) and (l) are merge of (d+e) and (j+k) with secondary staining for neuron-specific β-tubulin III. The white arrows indicate co-localization of IL-1 type 1 receptor or P-STAT3-Tyr705 with neuron-specific staining. The bar = 100 μm. (m) Western blot for IL-1 type 1 receptor comparing control and treated with IL-1β cultures for 30 minutes in vitro with quantification. Expression levels are expressed relative to T-ERK and are means ± SEM (n = 3 replicates); no significant difference between Ctrl and +IL-1β.