Figure 2
From: Exosomes neutralize synaptic-plasticity-disrupting activity of Aβ assemblies in vivo
Exosomes abrogate ADDL-mediated disruption of LTP. (A) Prior infusion of exosomes (4 μg in 5 μl, asterisk) prevented ADDLs (10 pmol in 5 μl, hash) inhibiting LTP. Animals received sequential injections of exosomes and ADDLs before the application of HFS (arrow). Insets show representative traces at the color-matched time points. Calibrations: 1.5 mV and 10 ms for all traces. (B) Dose-dependent protective effect of exosomes against ADDL-mediated disruption of LTP (0 μg Exo + ADDL, 100 ± 3%, n = 5; 0.2 μg Exo + ADDL, 105 ± 5%, n = 4; 1 μg Exo + ADDL, 123 ± 5%, n = 4; 4 μg Exo + ADDL, 151 ± 10%, n = 6; 10 μg Exo + ADDL, 168 ± 9%, n = 4). Statistical significance was expressed as **, P < 0.01 comparing to control group injected with 0 μg Exo + ADDL. (C) Neither exosomes nor ADDLs (5 μl, asterisk) affected baseline excitatory synaptic transmission in the CA1 area in vivo (PBS, n = 4; ADDL, n = 5; Exo, n = 4). (D) Exosomes (4 μg in 5 μl, asterisk) did not enhance decremental or standard LTP. An arrow indicates the time point of application of either weak HFS for decremental LTP or HFS for standard LTP, respectively. Insets and calibrations as in A. Error bars, ± SEM.