Figure 3

CGCs and CNs show different ER stress responses to glycosylation disruption induced by PMM2 downregulation or TM treatments. CGCs and CNs were infected with lentivirus containing PMM2 shRNA1 or scrambled control (SC) shRNA for 3 d (A), or treated with TM for 3 d (B). Equal amounts of cell lysate proteins were subjected to Western blotting with antibodies against different ER stress-response proteins. Protein levels were quantified by densitometry. C. Protein level changes upon PMM2 downregulation in A were determined in CNs and CGCs, respectively. D. Protein level changes upon treatment with 1 ng/mL TM in CGCs and upon 25 ng/mL TM in CNs in B were determined. The degree of protein level change within CGCs and CNs were compared to one (indicated by dashed lines), n = 3, *: p < 0.05; **: p < 0.01; ***: p < 0.001. The degree of protein level change between CGCs and CNs were also compared, n = 3, #: p < 0.05; ###: p < 0.001.