Figure 3
From: High glutamate permeability and distal localization of Best1 channel in CA1 hippocampal astrocyte
TFLLR-induced conductance is mediated by Best1 channel in hippocampal CA1 astrocytes. A. Representative current responses induced by whole-cell patch clamp from HEK293T expressing hSK1 using KCl-containing pipette solution (n = 8). The current responses were recorded in response to a voltage ramp command (from -100 to +100 mV, 1 s duration, 0.2 Hz; Vh of -60 mV. Internal pipette solution is composed of (mM) 150 KCl, 10 HEPES, 1 CaCl2, 1 MgCl2, and 5 EGTA (100 nM free Ca2+). B. The 5 selected representative current–voltage relationships recorded from a). C. Representative current responses induced by whole-cell patch clamp from HEK293T expressing hSK1 using KCl-containing pipette solution (n = 7). D. The 5 selected representative current–voltage relationships recorded from c). Averaged time that Er reaches to plateu from rupture is 63.4 ± 14.6 s (n = 7).