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Table 1 qRT-PCR validation of genes selected from fold change analysis of microarray data in the striatum

From: Involvement of the N-methyl-d-aspartate receptor GluN2D subunit in phencyclidine-induced motor impairment, gene expression, and increased Fos immunoreactivity

Symbol

Definition

n

KOS/WS (vs. WS) %

WPCP/WS (vs. WS) %

KOPCP/KOS %

(vs. WPCP/WS)

Significantly changed gene expressions of NR2D KO mice

Slc17

vesicular glutamate transporter 2

4

210 ± 39 *

117 ± 14

49 ± 12

c

Hace1

HECT domain and ankyrin repeat containing, E3 ubiquitin protein ligase 1

7

157 ± 12 **

101 ± 9

84 ± 5

a

Smpd4

sphingomyelin phosphodiesterase 4 (FLJ20297)

6

147 ± 4 **

105 ± 7

86 ± 5

b

Osbpl8

oxysterol binding protein-like 8 (ORP8), transcript variant 1

4

136 ± 8 **

112 ± 10

68 ± 7

c

Significantly changed gene expressions induced by PCP of wild-type mice

Fos

FBJ osteosarcoma oncogene

4

110 ± 8

283 ± 40 *

109 ± 12

c

Txnip

thioredoxin interacting protein (Txnip), transcript variant 1

4

137 ± 5 *

211 ± 29 **

122 ± 11

b

Pglyrp1

peptidoglycan recognition protein 1 (PGRP-S)

4

120 ± 5

176 ± 7 **

126 ± 6

c

Fosb

FBJ osteosarcoma oncogene B

3

122 ± 3

162 ± 29 **

74 ± 9

b

Sesn1

sestrin 1

4

116 ± 6

155 ± 11 **

120 ± 7

b

Arrdc

arrestin domain containing 3 (mKIAA1376)

4

133 ± 8 **

149 ± 10 **

95 ± 9

c

Zbtb4

zinc finger and BTB domain containing 4

4

115 ± 5

141 ± 3 *

109 ± 6

c

Bag-3

Bcl2-associated athanogene 3

4

107 ± 3

132 ± 5 **

108 ± 7

b

Ddit4

DNA-damage-inducible transcript 4 (REDD1)

4

124 ± 10

184 ± 27 **

142 ± 19

a

Gadd45g

growth arrest and DNA-damage-inducible 45 γ

4

119 ± 5

170 ± 15 *

126 ± 26

a

Tsc22d3

TSC22 domain family, member 3, transcript variant 1 (DSIPI)

4

100 ± 4

154 ± 18 *

148 ± 17

a

Cbr3

carbonyl reductase 3

4

102 ± 5

152 ± 6 **

149 ± 17

a

Cfp

complement factor properdin (Properdine)

4

133 ± 34

142 ± 14 *

126 ± 34

a

Sbf1

SET binding factor 1

6

91 ± 10

141 ± 14 *

217 ± 65

a

Slc2a1

solute carrier family 2 (GLUT1)

4

103 ± 8

140 ± 10 **

136 ± 14

a

Sgk1

serum/glucocorticoid regulated kinase 1

4

120 ± 11

138 ± 9 **

114 ± 16

a

Rgc32

RIKEN cDNA 1190002H23 gene

4

104 ± 4

133 ± 7 *

125 ± 12

a

Egr1

early growth response 1(zif-268)

4

114 ± 11

60 ± 5 *

57 ± 10

a

Significantly changed gene expressions induced by PCP of GluN2D KO mice

Egr4

early growth response 4

4

121 ± 9

 82 ± 10

 48 ± 4

b

  1. Data are expressed as the mean percentage ± SEM relative to the saline treated controls.
  2. Compared to WS controls; *, p <0.05. **, p <0.01.
  3. WPCP/WS vs. KOPCP/KOS; a, not significant. b, p <0.05. c, p <0.01.
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Molecular Brain

ISSN: 1756-6606