Astrocyte-specific expression of the GST-IP
sponge. (A) Tg1, Tg2 mouse lines were generated. In the DTg mice, expression of the GST-IP3sponge and nls-lacZ is induced under the control of TetO, but can be suppressed by Dox. (B, C) Whole mount lacZ staining of sagittal brains of Tg1 (B) and DTg (C) mice. (D, E) LacZ staining of coronal hippocampal slices of Tg1 (D) and DTg (E) mice. The lacZ expression level in Tg1 was very low (B), but hippocampal dentate gyrus cells showed some leakage (D). Scale bar, 0.5 mm. (F, G) Double immunofluorescence analysis of hippocampal CA1 of DTg mice using antibodies to lacZ, S100B, and NeuN. Insets are magnified images. Scale bar, 100 μm. (H – J) Double immunofluorescence analysis using antibodies to lacZ and GST. Scale bar, 25 μm.