Figure 1

Representative raw spectra from WT flies and flies lacking expression of, and overexpressing, PDF. A) Spectra acquired from WT flies included many well resolved peaks with ion signal at m/z 1972.0 (inset, [M + H]⁺ of PDF) generally resolvable, although often with low S/N. B) Spectra acquired from PDF-null flies were similarly rich in features, but without discernible ion signal at m/z 1972.0 (inset). C) Spectra acquired from PDF overexpressing flies, while of overall similar quality to those obtained from WT flies, had the ion signal at m/z resolved with far greater S/N.