|
Obs. m/z(Calc. m/z)
|
Precursor (UniProtKB Entry name)
|
Peptide name
|
Peptide sequence
|
Previous ref.
|
|---|
|
925.481* (925.436)
|
FMRF_DROME
|
PDNFMRFamide
|
R.PDNFMRFa.G
|
[1, 2, 7, 9, 30]
|
|
974.592 (974.589)
|
SNPF_DROME
|
RLRF peptide 2
|
R.SPSLRLRFa.G
|
[1, 2, 9, 18, 19, 30]
|
|
1182.578 (1182.573)
|
FMRF_DROME
|
DPKQDFMRFamide (FMRFamide 2)
|
R.DPKQDFMRFa.G
|
[1, 2, 7, 9, 19, 30]
|
|
1247.658 (1247.653)
|
NEMS_DROME
|
Dromyosuppressin (TDVDHVFLRFamide)
|
R.TDVDHVFLRFa.G
|
[1, 2, 7, 9, 18, 19, 30]
|
|
1395.748 (1395.753)
|
Q59E62_DROME
|
SIFamide (IFa-1)
|
A.AYRKPPFNGSIFa.G
|
[1, 2, 9, 18, 19]
|
|
1430.745 (1430.754)
|
CP2B_DROME
|
CAP-3AA2-AA15
|
T.GPSASSGLWFGPRLa.G
|
[1, 2, 9, 19, 30]
|
|
1471.791 (1471.773)
|
NPLP1_DROME
|
MTYamide peptide [NPLP1 (MTY)]
|
R.YIGSLARAGGLMTYa.G
|
[1, 2, 7, 9, 18, 19]
|
|
1531.812 (1531.802)
|
CP2B_DROME
|
CAP-3 (CAPA-3)
|
R.TGPSASSGLWFGPRLa.G
|
[1, 2, 9, 19, 30]
|
|
1534.819 (1534.834)
|
NPLP1_DROME
|
NPLP1-4 [NPLP1 (VQQ)]
|
R.NLGALKSSPVHGVQQ.K
|
[1, 2, 18, 19]
|
|
1653.907 (1653.908)
|
NPLP1_DROME
|
IPNamide [NPLP1 (IPN)]
|
R.NVGTLARDFQLPIPNa.G
|
[1, 2, 7, 9, 18, 19, 30]
|
|
1972.015 (1972.017)
|
PDF_DROME
|
Neuropeptide PDF
|
R.NSELINSLLSLPKNMNDAa.G
|
[19]
|
|
1991.044 (1991.043)
|
NPLP1_DROME
|
NPLP1-3AA1-AA18 [QRAamide]
|
R.NVAAVARYNSQHGHIQRAa.G
|
Novel
|
|
2094.091 (2094.088)
|
NPLP1_DROME
|
NPLP1-2 [NPLP1 (DPK)]
|
R.NIATMARLQSAPSTHRDPK.R
|
Novel
|
|
2249.130 (2249.128)
|
NPLP1_DROME
|
NPLP1-3 [NPLP1 (GAE)]
|
R.NVAAVARYNSQHGHIQRAGAE.K
|
Novel
|
- Listed are D. melanogaster neuropeptides identified in this study. The final three listings are believed to be novel. Reported observed m/z’s are the average of the m/z’s observed between the two replicate experiments MALDI-TOF MS experiments described. All observed m/z’s listed were determined to be monoisotopic [M + H]+’s of listed neuropeptides based on the calculated monoisotopic [M + H]+ (shown in parentheses below observed m/z). Truncated peptides are denoted by superscripts showing amino acids present from the annotated peptide sequence (i.e. NPLP1-3AA1-AA18 is missing the final three residues of the annotated NPLP1-3 sequence, also shown). Peptide sequences include pre- and post- cleavage residues separated from sequences by a period. C-terminal amidation is denoted by an “a” at end of the peptide sequence. Abbreviations are listed in Table 2 legend. *m/z 925.481 likely corresponds to the convolution of PDNFMRFamide (monoisotopic [M + H]+ = 925.435) and Drostatin-3 (Ast-A3, monoisotopic [M + H]+ = 925.489, see Table 2), which were detected as separate peaks with MALDI-FTICR-MS (Figure 6), hence the comparatively larger m/z error.
