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Table 1 Peaks observed in MALDI-TOF MS profile spectra confirmed to be neuropeptides by MALDI-TOF/TOF MS/MS fragmentation

From: A rapid MALDI-TOF mass spectrometry workflow for Drosophila melanogaster differential neuropeptidomics

Obs. m/z(Calc. m/z) Precursor (UniProtKB Entry name) Peptide name Peptide sequence Previous ref.
925.481* (925.436) FMRF_DROME PDNFMRFamide R.PDNFMRFa.G [1, 2, 7, 9, 30]
974.592 (974.589) SNPF_DROME RLRF peptide 2 R.SPSLRLRFa.G [1, 2, 9, 18, 19, 30]
1182.578 (1182.573) FMRF_DROME DPKQDFMRFamide (FMRFamide 2) R.DPKQDFMRFa.G [1, 2, 7, 9, 19, 30]
1247.658 (1247.653) NEMS_DROME Dromyosuppressin (TDVDHVFLRFamide) R.TDVDHVFLRFa.G [1, 2, 7, 9, 18, 19, 30]
1395.748 (1395.753) Q59E62_DROME SIFamide (IFa-1) A.AYRKPPFNGSIFa.G [1, 2, 9, 18, 19]
1430.745 (1430.754) CP2B_DROME CAP-3AA2-AA15 T.GPSASSGLWFGPRLa.G [1, 2, 9, 19, 30]
1471.791 (1471.773) NPLP1_DROME MTYamide peptide [NPLP1 (MTY)] R.YIGSLARAGGLMTYa.G [1, 2, 7, 9, 18, 19]
1531.812 (1531.802) CP2B_DROME CAP-3 (CAPA-3) R.TGPSASSGLWFGPRLa.G [1, 2, 9, 19, 30]
1534.819 (1534.834) NPLP1_DROME NPLP1-4 [NPLP1 (VQQ)] R.NLGALKSSPVHGVQQ.K [1, 2, 18, 19]
1653.907 (1653.908) NPLP1_DROME IPNamide [NPLP1 (IPN)] R.NVGTLARDFQLPIPNa.G [1, 2, 7, 9, 18, 19, 30]
1972.015 (1972.017) PDF_DROME Neuropeptide PDF R.NSELINSLLSLPKNMNDAa.G [19]
1991.044 (1991.043) NPLP1_DROME NPLP1-3AA1-AA18 [QRAamide] R.NVAAVARYNSQHGHIQRAa.G Novel
2094.091 (2094.088) NPLP1_DROME NPLP1-2 [NPLP1 (DPK)] R.NIATMARLQSAPSTHRDPK.R Novel
2249.130 (2249.128) NPLP1_DROME NPLP1-3 [NPLP1 (GAE)] R.NVAAVARYNSQHGHIQRAGAE.K Novel
  1. Listed are D. melanogaster neuropeptides identified in this study. The final three listings are believed to be novel. Reported observed m/z’s are the average of the m/z’s observed between the two replicate experiments MALDI-TOF MS experiments described. All observed m/z’s listed were determined to be monoisotopic [M + H]+’s of listed neuropeptides based on the calculated monoisotopic [M + H]+ (shown in parentheses below observed m/z). Truncated peptides are denoted by superscripts showing amino acids present from the annotated peptide sequence (i.e. NPLP1-3AA1-AA18 is missing the final three residues of the annotated NPLP1-3 sequence, also shown). Peptide sequences include pre- and post- cleavage residues separated from sequences by a period. C-terminal amidation is denoted by an “a” at end of the peptide sequence. Abbreviations are listed in Table 2 legend. *m/z 925.481 likely corresponds to the convolution of PDNFMRFamide (monoisotopic [M + H]+ = 925.435) and Drostatin-3 (Ast-A3, monoisotopic [M + H]+ = 925.489, see Table 2), which were detected as separate peaks with MALDI-FTICR-MS (Figure 6), hence the comparatively larger m/z error.