Figure 2

Generation of iN cells. (A, B) Transgene-dependent conversion of common marmoset fibroblasts into neuronal cells, in which synapsin reporter activation and morphological changes were dependent on the exogenous transcription factor and time. Cells became synapsin reporter-positive accompanied by a gradual morphological changes into neuronal cells, both effects of which were not found in cells cultured in neural medium without dox at 21 div. (B) Magnified images of Figure 1A showing synapsin reporter-positive cells with morphological changes from fibroblasts to neuronal cells. (C) Counts of synapsin reporter-positive cells with neuronal morphology showed a dox-dependent increase in their number (positive cells with fibroblast morphology were excluded from the counts). P^*** < 0.001, P^## < 0.01 (one-way ANOVA followed by Tukey’s test). (D-F) The effect of sustained dox treatment on the production of cjiN cells. (D) Cells were treated with dox at 1 div until 3, 5, 7, 9 or 11 div and then maintained without dox until 23 div. (E) Synapsin reporter-positive cells with neuronal morphology were observed in all treatment group. (F) Cell counts revealed that a longer treatment time of dox increased the number of cjiN cells. Although dox treatment from 1–3 div sufficiently promoted cjiNs, a longer treatment time increased their number. P^*** < 0.001 (one-way ANOVA followed by Tukey’s test). Scale bar; 200 μm.