Table 1 Primer sets used in RT-PCR analysis
From: Reprogramming non-human primate somatic cells into functional neuronal cells by defined factors
Gene name | Forward primer | Reverse primer | Product size (bp) |
|---|---|---|---|
SYN1 | acggagactaccgcagtttg | cgatctgctccagcattgca | 459 |
DCX | ctgtgcgtgtgcttctgaac | tcagctggagacttgcttcg | 346 |
TUBB3 | catagaccccagtggcaactacg | caccctccgtgtagtggcccttgg | 241 |
MAP2 | cctgtgttaagcggaaaacc | agagactttgtcctttgcctgt | 86 |
VGLUT1 | tcaataacagcacgacccac | tcccggaatttgagtgacaatg | 131 |
SCN1A | attggcaattccgtgggagc | cccacacagcacgcggaaca | 204 |
GRIN1 | accccaagatcgtcaacatcg | ggctaaccagaatggcgtaga | 213 |
GRIA1 | cgagctttcctgttgatacat | tctgccacttgtaatggtcgatg | 99 |
endo BRN2 | aattaaggaaaaaggaaagcaact | caaaacatcattacacctgct | 71 |
endo NEUROD1 | gttattgtgttgccttagcacttc | agtgaaatgaattgctcaaattgt | 77 |
ACTB | ggcatccacgaaactaccttt | acactgagtacttgcgctcg | 202 |