Figure 1

Pharmacological blockade of TRPV1 activation with AMG9810 does not affect the LTP induction in the ACC. (A and B) An overview of 64-channel multi-electrode array recordings in one AMG9810-treated ACC slice (A: before TBS; B: 2 h after TBS). After stabilizing the baseline responses for 20 min, AMG9810 (10 μM) was bath applied from 20 min before till 20 min after delivery of TBS to the deep layer. It could be apparently seen that AMG9810 infusion could not prevent the LTP induction. Red circles indicate the stimulated channel (Ch. 29). Vertical lines demarcate the different layers in the ACC. (C) Results of one LTP-showing channel (Ch. 38 and Ch. 30) from the superficial layer of one slice for control and AMG9810-treated group, respectively. (D) Summary of averaged data from 5 superficial layer channels of one slice for both control and AMG9810-applied group, respectively. (E) Pooled data of the superficial layer of the ACC from 6 slices from 6 mice. (F) Pooled data in the deep layer of the ACC (n = 5–6 slices/5-6 mice). TBS delivery results in an enduring synaptic potentiation that lasts for at least 2 h, which is not affected by the presence of AMG9810. Insets in (C and F): representative fEPSP traces at time points indicated by the numbers in the graph. Arrows in (C-F) denote the starting point of TBS application. Calibrations in (A, C and F): 100 μV, 10 ms. Error bars in (E and F) represent SEM.