Figure 3

Alterations in microglial reaction after spinal cord ischemia reperfusion (I/R) injury. (A) Effects on the morphology changed in microglia after spinal cord after I/R injury. Scale bars are 200 μm in 3A a, c; 50 μm in 3A-b, d; (B) Effects on spinal immunoreactivity to Iba-1 after I/R injury. Scale bars are 200 μm. (C) Quantification of Iba-1–positive cells in the spinal cord’s dorsal horn is presented as mean ± SEM (n = 6 per group).Prominent Iba-1 positive cells activated by I/R injury were observed in both spinal dorsal horns of rats subjected to the surgical operation, who exhibited hypertrophic morphology with thick processes. Increases in Iba-1 immunoreactivity and number of Iba-1–positive cells in spinal dorsal horn were markedly attenuated by intrathecal injection of minocycline, TAK-242 and PDTC but was synthetically activated by LPS. **P < .01 compared to Sham group; ^##P < .05 compared to I/R group.