Figure 3

Functional assays of LRRK2 and LC1 interaction. (A) MTT assay of SKNSH transiently expressing LRRK2-WT or its mutants alone (black columns) or co-expression with LC1 (grey columns). Experiment was performed in triplicates and repeated three times. Data represent mean values with standard error bars. (B) Apoptosis assay of SKNSH transiently expressing LRRK2 or its mutants alone (black columns) or co-expression with LC1 (grey columns). Experiment was carried out with six replicates and data in the bar graph represent mean values with standard error bars. Statistical significance was determined using Student’s t-test and *p < 0.05, **p < 0.01 and ***p < 0.001.