Cre expression and GABRA 4 KO in the transfected MD cells. (A and B) Opposite to the MD cells from the mice injected with AAV-mCherry, immunofluorescence revealed Cre expression (green) in the mCherry-expressing cells in the MD region of the mice injected with AAV-mCherry-Cre. (C) A decrease of GABRA4 expression can be seen in the injected side of the MD in the Cre virus-injected mice compared with the contralateral non-injected side or the control virus-injected mice. Arrows indicate the injection sites. Scale bars: 500 μm (top), and 250 μm (bottom).