Figure 1

The expression of Fos protein was upregulated in different brain regions in zymosan-treated mice. (A) Immunohistochemical staining for Fos in the ACC (anterior cingulate cortex, 0.98 mm prior to bregma), PFC (prefrontal cortex, 1.78 mm prior to bregma), IC (insular cortex, 1.18 mm prior to bregma), Amy (amygdala, 1.46 mm posterior to bregma), PAG (periaqueductal gray, 4.72 mm posterior to bregma), PBN (parabrachial nucleus, 5.20 mm posterior to bregma) and NTS (nucleus of solitary tract, 7.76 mm posterior to bregma) at day 7 after intracolonic injection of zymosan or saline (control). (CeA: central amygdala; cc: central canal; Scale bar: ACC, PFC and IC, 250 μm; Amy and PAG, 150 μm; PBN and NTS, 100 μm). (B) Histogram showing the quantification of Fos-immunoreactive (Fos-ir) neurons in the above brain regions of saline- and zymosan-treated mice (n = 6 slices per mouse; 6 mice per group). * denotes a significant difference between zymosan- and saline-treated mice.