Figure 1

LBs are specifically localized in GABAergic cortical neurons. A) Broad image of the specific localization of LBs are only in GABAergic cortical neurons in paraffin sections of temporal cortex of EPM2A-/- mice (3 months-old) revealed with DAB. The cortical neuronal marker used in the immunostaining is the GAD67. B) Magnification of the picture 3A (left) of LBs located in the cytoplasm and combination with PAS staining (right). C) Immunofluorescence of GAD67 (green), polyglucosan antibody (red) and DAPI staining (blue) in wild type (left) and EPM2A-/- mice (right). These results showed that the specific localization of LBs exclusively in the GABAergic neurons at early ages gives us a new perspective about the sensible damage induced in these neurons due to laforin deficiency.