Figure 2
From: Loss of GABAergic cortical neurons underlies the neuropathology of Lafora disease
Decrease of GABAergic neurons density in laforin-deficient mice. We measured by different techniques the decrease of GABAergic cortical neurons in EPM2A-/- mice (1 month-old) (*P < 0.05). A) Immunohistochemistry performed in paraffin section of temporal cortex revealed with DAB, showing a strong reduction of GABAergic cortical neurons. Cell counting of GABAergic cortical neurons in 15 days-old and 1 month-old EPM2A-/- mice (n = 3) shows a significant reduction of 54.70% in the number of GAD67 neurons in EPM2A-/- compared with wild-type, which suggest the importance of the temporary window between 15 days and 1 month of age. B) Immunofluorescence and confocal study (n = 3) corroborating the same results. C) Cerebral cortex lysates measured by western blot (n = 5). This gel shows a clear GAD67 reduction in the cerebral cortex of 1 month old laforin-deficient mice.