Chronic visual loss does not affect aggressiveness of single-housed flies that lack social experience prior to behavioral tests. (A) The level of aggressiveness (i.e. total aggression) was quantified by counting the number of all aggressive events (i.e. lunges, wing threats, tussles, boxing, and holding) within 10-min period. No significant difference was observed between single-housed wild type and ninaB1 mutants, or between single-housed ninaB1 mutants and rescued individuals in which vision was restored by eye-specific expression of a ninaB transgene in ninaB1 mutants. Pairs of flies tested: CS, n = 27; ninaB1 Rescue, n = 26; ninaB1, n = 22. (B) Loss of eye in eya2 mutants did not affect aggressiveness of single-housed flies. The level of aggressiveness of single-housed eya2 mutants was comparable to that of wild-type or eya2 heterozygous flies. Pairs of flies tested: CS, n = 27; eya2/+, n = 20; eya2, n = 20. ns, not significant (p > 0.05). Error bars represent SEM.