Changes in autophagic factors after acute LPS-induced inflammatory stress. Representative immunoblots showed the immunoreactivity of Beclin-1 (A), p62 (B) LC3 II (C) in cortex and Beclin-1 (D), p62 (E) LC3 II (F) in hippocampus from mice treated with a single () or two () or three () i.p. injections of LPS at 10mg/kg or vehicle (0.9% NaCl). Mice were sacrificed 2, 4, 6 or 12 h after a single injection and 24 h after two and three injections. Semi-quantitative analysis of immunoblot was performed using Gene Tools software (Syngene, Ozyme France). The immunoreactivity of protein was normalized to β-tubulin immunoreactivity. The results are expressed as arbitrary units (% of 0.9% NaCl-injected mice group set at 100%). Results are mean±SEM for 6 mice in each group. *p < 0.05, **p < 0.01 compared to 0.9% NaCl-injected mouse group by Kruskal-Wallis test with a Dunns multiple comparison test.