Figure 7

Changes in autophagy markers after chronic LPS-induced inflammatory stress. Representative immunoblots showed the immunoreactivity of Beclin-1 (A), p62 (B) and LC3 II (C) in cortex and hippocampus from LPS-treated mice (0.5mg/kg of LPS every 3 days for 3 months) or control (0.9% NaCl every 3 days for 3 months). Treatment started at 3 months and mice were sacrificed at 6 months of age. Densities were quantified by using Gene Tools software (Syngene, Ozyme France). Data of each protein were reported to data of the corresponding β-tubulin. The results are expressed as arbitrary units (% expression of 0.9% NaCl-treated mice set at 100%). Results are mean±SEM for 6 mice in each group. ^*p < 0.05, ^**p < 0.01 compared to control mice by Mann-Whitney test.