Figure 11
From: Histamine impairs midbrain dopaminergic development in vivo by activating histamine type 1 receptors
High doses of chlorpheniramine affect normal embryonic development but neither chlorpheniramine nor cimetidine affects dopaminergic neurons. (A) Micrographs from E14 rat embryos injected at E12 with different doses of the H1R antagonist, chlorpheniramine. Fifty and twenty-five micrograms interfered with normal development, while 15 μg allows normal development of the embryo. (B) TH and β-III Tubulin staining in coronal sections of the VM from E14 rat embryos injected with 15 μg of the H1R antagonist, chlorpheniramine or 50 μg of the H2R antagonist, cimetidine. The staining patterns were not modified by these antagonists. Scale bar: 150 μm.