Figure 6

The regulation of spine ER dynamics by STEP. (A) Scatter plot showing correlation between spine ER content and spine volume at t = 0 min for WT. Equivalent plot for (B) STEP KO and (C) STEP KO pre-treated for 10 min with L689,560. (D) Cumulative probability plot of ER volume at t = 0 for WT, STEP KO and STEP KO with L689,560 (K-S test, P < 0.001 between WT and STEP KO and P < 0.001 between STEP KO and STEP KO + L689,560). (E) Scatter plot for changes in the spine ER content as a function of changes in spine volume over the 10 min period for WT, treated with vehicle. Equivalent plots for (F) STEP KO and (G) STEP KO with L689,560. (H) Cumulative probability plot of normalised spine volumes (K-S test, P < 0.001 between WT and STEP KO or between STEP KO and STEP KO + L689,560). (I) Changes in ER volume for the three groups in growing spines (Mann–Whitney, P < 0.001 between WT and STEP KO and P < 0.05 between STEP KO and STEP KO + L689,560). (J) Changes in ER volume for the three groups in shrinking spines.