Phase contrast pictures (20 X magnification) showing the gross morphology of the cells at different time points of the culture. Rounded cell bodies can be seen soon after plating the cells. Discrete cell clumps are visible at the early time points of the culture. Significant proportions of cells at this time point represent NPCs, which later on give rise to neurons and astrocytes. Abundant numbers of cells are present even at DIV40 of the culture. The insets are representative portions from the same image that have been enlarged.