Figure 3

ICC study of the culture using neuronal and glial-specific marker shows the presence of both the phenotypes in the culture. Interestingly, cells at the initial period of the culture are labeled with both neuronal and glial markers indicating newly generated or immature cells. However, the cells become mature at the later time points (DIV 20-24), when neurons and glia are stained separately by pan-neuronal and GFAP antibodies, respectively.