Prevention by MgT treatment of impairment in NMDAR signaling pathway in APPswe/PS1dE9 transgenic mice (Tg mice). (A) Representative Western blots showing the expression levels of NR2B, pCaMKII, CaMKII, pCREB, and CREB in the hippocampus of mice sacrificed either after home-cage conditions (basal) or following 24 h of environmental enrichment-based behavioral stimulation (Stim). GAPDH expression served as loading control. (B) Left: Quantitative analysis of NR2B expression in the hippocampus of WT, Tg and Tg + MgT mice (n = 5/group), obtained by Western blot (arbitrarily units, a.u.). Mice were sacrificed under basal conditions without behavioral stimulation. Right: Quantitative analysis of NR2B expression the hippocampus of WT (n = 6), Tg (n = 6) and Tg + MgT mice (n = 5) obtained by Western blot following behavioral stimulation. (C and D) same as (B) but quantifying Phosphorylated CaMKII (pCaMKII)/CaMKII ratio (C) and Phosphorylated CREB (pCREB)/CREB ratio (D). ANOVAs (Table2) were followed by Bonferroni's post hoc test. Error bars show SEM. * p < 0.05, *** p < 0.001.