Figure 1
From: A mouse line for inducible and reversible silencing of specific neurons
Gene targeting of the IVMR-2A-GFP sequence into the ROSA26 locus by homologous recombination. (a) Diagram of the targeting construct and the expected recombination events. CAG, CAG promoter; WPRE, the woodchuck hepatitis virus posttranscriptional regulatory element; pA, polyA sequence; PGK, PGK promoter; NeoR, neomycin resistance positive selection cassette; DTA, diphtheria toxin negative selection cassette. (b) 5'arm and 3'arm PCR analysis showing the identification of positive targeted ES clones (Lanes 2, 4, 5, 7 and 10). (c) Genotyping of mouse pups to identify offspring harboring the conditional allele.