Figure 4

IVM-induced behavioral alteration in free moving Rosa26-IVMR mice. (a) Rotation towards AAV2-Cre virus-injected side is only present in Rosa26-IVMR mice treated with both apomorphine and IVM but not those treated with one of them. Wild type controls do not show any rotation when treated with both of them or either of them. Apomorphine-induced rotation was performed 12 h after administration of IVM (10 mg/kg). n = 6 for wild type; n = 8 for Rosa26-IVMR mice; ***P < 0.001, two-way repeated ANOVA test. (b) Time course of apomorphine-induced rotation in Rosa26-IVMR mice with unilateral injection of AAV2-Cre virus. n = 9 for each; **P < 0.01 (5 mg/kg vs 10 mg/kg group), two-way repeated ANOVA test. (c) Dose effect of IVM on apomorphine-induced rotation in Rosa26-IVMR mice at 12 h and 1 d post systemic administration. Six to eleven mice were included in each set of experiments; **P < 0.01, one-way ANOVA test. (d) Apomorphine-induced rotation is similar between the first- and second cycles of IVM administration. This observation was done 1 d after IVM treatment (10 mg/kg). n = 6 for each; P = 0.7, paired samples test. (e) Thermal sensation shown by the tail immersion test is lowered in Rosa26-IVMR;Prrxl1-Cre^ER mice 1 d after administration of IVM (10 mg/kg), but unchanged in wild type mice. n = 11 for wild type, n = 13 for Rosa26-IVMR;Prrxl1-Cre^ER mice; *P < 0.05, two-way repeated ANOVA test. (f) Mechanical sensation shown by the von Frey test is decreased in Rosa26-IVMR;Prrxl1-Cre^ER mice at 1 d and 2 d after systemic administration of IVM, but unchanged in wild type mice. n = 11 for wild type, n = 13 for Rosa26-IVMR;Prrxl1-Cre^ER mice; *P < 0.05, **P < 0.01, two-way repeated ANOVA test.