Figure 4
From: Inhibition of phosphodiesterase10A attenuates morphine-induced conditioned place preference
MP-10 (2.5 mg/kg) causes long-lasting changes in expression of pCREB immunoreactivity. (A) Immunofluorescence images of pCREB in different brain areas from rats receiving saline (Sal, 2 ml/kg, s.c.), morphine (Mor, 10 mg/kg, s.c.), MP-10 (MP-10, 2.5 mg/kg, s.c.) or a combination of both drugs (MP-10/Mor). MP-10 was administered 30 min prior to morphine and after CPP testing (scale bar represents 50 μm). (B) Quantification of pCREB positive nuclei. Data are expressed as mean±SEM and analyzed by two-way ANOVA followed by Bonferroni posttests. *P < 0.05 when comparing between morphine and saline groups. #P < 0.05 when comparing the morphine group to the drug combination group. N = 5 per group. DMS: dorsomedial striatum; CORE: NAc core; SHELL: NAc shell; ACC: anterior cingulate cortex.