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Figure 9 | Molecular Brain

Figure 9

From: Sensitization of neurons in the central nucleus of the amygdala via the decreased GABAergic inhibition contributes to the development of neuropathic pain-related anxiety-like behaviors in rats

Figure 9

Detection of apoptosis in the CeA nucleus using TUNEL staining and caspase-3 western blot. (A): TUNEL staining. (a) and (b): DAPI staining for marking the nucleus of cells (blue); (c) and (d): TUNEL staining for marking the apoptotic cells (red). Note that not any legible apoptotic cells are observed on the CeA tissue either in SNL or in sham-operated rats. Scale bar = 100 μm, n = 9 tissues from 3 rats/group. (B): positive apoptotic cells control. The amygdala and hippocampal tissues are treated with DNase I recombinant (1000 U/ml) to induce positive apoptotic cells. (a) and (b): DAPI staining for marking the nucleus of cells (blue); (c) and (d): TUNEL staining for marking the apoptotic cells (red). Note that after cells were treated by DNase I, a large numbers of legible apoptotic cells are found in the amygdala and the hippocampal tissues. Scale bar = 100 μm, n = 9 tissues from 3 rats. (C): Western blot assay of caspase-3 expression in the CeA nucleus. Upper: representative of Western blot bands; Lower: analysis of the relative intensity of caspase-3. β-actin is used as an internal control. No significant difference is observed on caspase-3 expression in the CeA nucleus among na''ve, sham and SNL rats. n = 6/group.

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Molecular Brain

ISSN: 1756-6606