NMDAR-LTD and depotentiation are inhibited by p110β Overexpression in the hippocampal CA1 Area. (A) Representative images of Flag-tagged p110β or tdTomato expression in the mouse hippocampal CA1 area. (Blue: DAPI, Green: Flag-p110β, Red: tdTomato, imaged 50 days after AAV injection). Scale bar represents 500 μm. (B, C) Input/output relationship was unaltered in p110β-overexpressed mice (green symbols) compared to control group (tdTomato; red symbols). The stimulation intensity was varied by 3 μA increments, and the insets indicate representative traces (averages of 4 successive sweeps; scale bars: 0.5 mV, 10 ms in this and the following panels). (D) Paired-pulse facilitation (PPF) in p110β mice was unaffected; the measured inter-stimulus intervals were 20, 50, 100 and 200 ms. (E) Excessive p110β had no effect on LTP induced by a brief tetanus (100 Hz, 1 s). Typical traces are obtained at the times indicated by a (light trace) and b (thick trace). (F) NMDAR-LTD induced by LFS (1 Hz, 15 min) was impaired in p110β mice (p <0.01, Student s t-test). (G) Depotentiation induced by LFS (2 Hz, 10 min) was reduced in p110β mice (p <0.01, Student s t-test).