Figure 2From: The planar cell polarity protein Vangl2 bidirectionally regulates dendritic branching in cultured hippocampal neuronsEffect of various mutants of Vangl2 on spine morphology. (A) The expression pattern of endogenous Vangl2 in the cultured hippocampal neurons at 21 DIV. Vangl2 was colocalized with PSD-95. (B) Schematic representation of HA-tagged Vangl2, and the constructs of deletion mutants. Numbers indicate amino acid sequences. TM, transmembrane region; PkBD, Prickle binding domain. (C) Spine morphology. Cultured hippocampal neurons were visualized by immunostaining for pmEGFP-β-actin at 21 DIV. (D) Ratio of spine density in neurons transfected with each Vangl2 construct. Data are mean % value relative to the level with control HA vector and SEM from four independent primary cultures. *p <0.05 as assessed by one-way ANOVA.Back to article page