Figure 2

Immunofluorescent measurement of phagocytosis. A – Representative image of microglia incubated with fluorescent latex beads (green), immunolabelled for CD68 to identify the cell soma (red) and nuclei stained with DAPI (blue), scale bar represents 50 μm. B – Representative confocal micrograph to demonstrate bead internalization. Microglia were incubated with fluorescent latex beads (green), immunolabelled for Iba1 to visualize cytoplasmic volume (red), and nuclei stained with DAPI. Scale bar represents 25 μm, yellow line indicates the region projected orthogonally in C.