Genome-wide screen for modifiers of Na + /K + ATPase alleles identifies critical genetic loci

Talsma, Aaron D; Chaves, John F; LaMonaca, Alexandra; Wieczorek, Emily D; Palladino, Michael J

doi:10.1186/s13041-014-0089-3

Molecular Brain

Table 6 Single gene effects confirmed for ATPalpha alleles using RNAi

From: Genome-wide screen for modifiers of Na⁺/K⁺ATPase alleles identifies critical genetic loci

Cytological region	Gene	Putative function^#	ATPα Allele	Nature of interaction	Significance
21A1-21B1	Galectin	Galactoside binding [45]	CJ10	Suppressor	**
21A1-21B1	Galectin	“	CJ5	Suppressor	***
22 F4-22 F4	CG3528	Unknown	DTS1	Enhancer	*
22 F4-22 F4	CG3528		CJ10	Suppressor	*
22 F4-22 F4	CG3528		CJ5	Suppressor	*
27E-28B1	Ndae1	Na + driven anion exchanger [47]	CJ5	Enhancer	*
29B4-29E4	Glt	Cell surface glycoprotein [50]	CJ10	Suppressor	*
30C8-30C9	Ppk11	Sodium channel [52]	CJ5	Suppressor	**
31C-32E	FKBP59	Calcium channel regulator [53]	CJ5	Suppressor	***
31C-32E	FKBP59	“	DTS1	Enhancer	*
33A1-33A1	Vha100-5	ATPase, proton transport	DTS1	Enhancer	*
33A2-33A2	Esc	Histone methyltransferase component [74]	DTS1	Enhancer	***
33A2-33A2	Esc	“	CJ10	Suppressor	**
34E4-35B4	Dyrk2	Serine/Threonine kinase [55]	DTS1	Enhancer	*
34E4-35B4	Dyrk2	“	CJ5	Suppressor	***
37A2-37A4	Ham	Transcription factor [75]	DTS1	Suppressor	*
37A2-37A4	Ham	“	CJ5	Suppressor	**
37C1-37C1	Ddc	Amino acid decarboxylase [76]	CJ5	Suppressor	***
25 F1-36A1	CG5888	Toll 3 like Receptor	CJ10	Suppressor	**
25 F1-36A1	CG5888	“	CJ5	Suppressor	*
50C5-50C6	Stj	Voltage-gated calcium channel regulatory subunit [77],[78]	DTS1	Enhancer	****
50C5-50C6	Stj	“	CJ5	Enhancer	**
51D1-51D1	Cyp6a19	Cytochrome P450	CJ10	Suppressor	*
62E8-63B6	Spz5	Neurotrophin [61],[62]	DTS1	Suppressor	**
62E8-63B6	Spz5	“	CJ10	Suppressor	*
62E8-63B6	Rasp	Palmitoyl transferase [65],[66]	CJ5	Suppressor	***
63A3-63A3	FMRFaR	Neuropeptide receptor [79]	DTS1	Enhancer	**
63A3-63A3	FMRFaR	“	CJ10	Suppressor	**
63A3-63A3	FMRFaR	“	CJ5	Suppressor	****
64C2-64C5	Con	Homophilic cell adhesion [80]	DTS1	Suppressor	*
64C2-64C5	Con	“	CJ5	Suppressor	**
67A2-67D13	Aay	Predicted phosphoserine phosphatase	CJ10	Suppressor	*
67A2-67D13	Aay	“	CJ5	Suppressor	**
67B9-67B9	Uch-L5	26S Proteasome component [81]	DTS1	Enhancer	*
67D11-67D11	Scramb1	Phosphatidyl serine scramblase	CJ10	Suppressor	**
99B5-99B6	Dop1R2	Dopamine 1-like receptor [82],[83]	CJ5	Suppressor	***
99B6-99B6	Ppk21	Sodium channel	DTS1	Suppressor	**
99B6-99B6	Ppk21	“	CJ10	Suppressor	*
100B9-100B9	Ppk24	Sodium channel	DTS1	Suppressor	**
100B9-100B9	Ppk24	“	CJ5	Suppressor	*
100B9-100B9	Ppk24	“	CJ10	Suppressor	**
100C1-100C1	CG11340	Predicted chloride channel	DTS1	Suppressor	*
100C1-100C1	CG11340	“	CJ5	Suppressor	***
100C1-100C1	CG11340	“	CJ10	Suppressor	*

Many genes had an interaction with more than one allele, although some appear to be allele specific. RNAi knockdowns were compared with ATPalpha*, daGal4/+ controls. ^*p < 0.05, ^**p < 0.01, ^***p < 0.001, ^****p < 0.0001.
^#Function per flybase.org and/or listed citation.

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ISSN: 1756-6606

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