Figure 6
DRP1 inhibitor Mdivi contracts Nlrx1 protective effect after rotenone treatments. (A) Quantification of released LDH assay after treatments with 100 μM rotenone and 50 μM Mdivi for 24 hours, n = 3. (B) Representative Immunoblotting of HMGB1 and HSP90 in cell culture supernatants after rotenone and Mdivi treatments. (C) Representative flow cytometry plots of KI and KD cells treated with 10 μM rotenone and 50 μM Mdivi. (D) Quantifications of three independent experiments. (E) Graphical representation of ratios of necrotic/late apoptotic cells to early apoptotic cells. * Significantly different from Sc, p < 0.05.