Neural and cell cycle-related miRNAs are altered in G93A-SOD1 mouse brain as disease progresses. RT-PCR analysis of brain-specific (A) and cell cycle-related (B) miRNAs in total RNA extracted from whole brain of G93A-SOD1, B6.SJL and Wt-SOD1 mice, at postnatal weeks 8 and 18 (ten mice per group). Each point represents a single brain. Relative expression data are presented as means ± SD. *p < 0.05; **p < 0.01; limma moderated t-test.