Figure 7
A combination of subthreshold synaptic stimulation and Best1-mediated astrocytic glutamate produces NMDAR-dependent LTP at SC-CA1 synapses. A, Summary of LTP recordings in hippocampal slices when subthreshold stimulation (40 Hz) with TFLLR (40 Hz + TFLLR; black circles), 40 Hz alone (40 Hz; white circles), TFLLR alone (TFLLR; gray circles), 40 Hz with TFLLR in the presence of APV (40 Hz + TFLLR + APV; green circles) or niflumic acid (40 Hz + TFLLR + NFA; red circles), and 40 Hz with endothelin (Endothelin; blue circles) was applied. B, Bar graph representing the averaged % baseline of fEPSPs (mean ± s.e.m.) over the time period indicated by the gray bar in A. *, p < 0.05, one-way ANOVA with post-hoc test. Same colors as A. Numbers of tested slices from at least three mice are indicated within each bar. C, Summary of LTP recording from loxP-floxed scrambled-shRNA expressing hippocampal slices (Sc-shRNA, black circles), loxP-floxed Best1-shRNA expressing slices from hGFAP-CreERT2 mice without [Best1-shRNA (−Tam), white circles] or with tamoxifen pretreatment [Best1-shRNA (+Tam), gray circles]. D, Bar graph representing the averaged % baseline of fEPSPs (mean ± s.e.m.) over the time period indicated by the gray bar in C. *, p < 0.05, one-way ANOVA with post-hoc test. Same colors as C. Numbers of tested slices from at least three mice are indicated within each bar. E, Summary of LTP recording from Sc-shRNA expressing hippocampal slices (black circles) or Best1-shRNA expressing slices from CaMKIIα-Cre mice (white circles). F, Bar graph representing the averaged % baseline of fEPSPs (mean ± s.e.m.) over the time period indicated by the gray bar in E. *, p < 0.05, unpaired t-test. Same colors as E. Numbers of tested slices from at least independent mice are indicated within each bar.