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Figure 1 | Molecular Brain

Figure 1

From: The novel protein kinase C epsilon isoform at the adult neuromuscular synapse: location, regulation by synaptic activity-dependent muscle contraction through TrkB signaling and coupling to ACh release

Figure 1

nPKCε and pnPKCε are expressed in adult and newborn skeletal muscle. (A) Western blot analysis of nPKCε and pnPKCε immunoreactivity in synaptic membrane in adult. (B) Western blot analysis of the novel PKC isoform ε was carried out to determine its presence in newborn (P6) and adult skeletal diaphragm muscle. Samples were also immunoblotted with an anti-phospho-PKCε antibody to identify specific phosphorylation of PKCε in resting conditions. The predicted molecular weight of the isoform is indicated. In all the experiments, we used actin as a loading control. The amount of protein loaded in each lane was 15 μg.

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Molecular Brain

ISSN: 1756-6606